TY - JOUR
T1 - A Twist2-dependent progenitor cell contributes to adult skeletal muscle
AU - Liu, Ning
AU - Garry, Glynnis A.
AU - Li, Stephen
AU - Bezprozvannaya, Svetlana
AU - Sanchez-Ortiz, Efrain
AU - Chen, Beibei
AU - Shelton, John M.
AU - Jaichander, Priscilla
AU - Bassel-Duby, Rhonda
AU - Olson, Eric N.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Skeletal muscle possesses remarkable regenerative potential due to satellite cells, an injury-responsive stem cell population located beneath the muscle basal lamina that expresses Pax7. By lineage tracing of progenitor cells expressing the Twist2 (Tw2) transcription factor in mice, we discovered a myogenic lineage that resides outside the basal lamina of adult skeletal muscle. Tw2+ progenitors are molecularly and anatomically distinct from satellite cells, are highly myogenic in vitro, and can fuse with themselves and with satellite cells. Tw2+ progenitors contribute specifically to type IIb/x myofibres during adulthood and muscle regeneration, and their genetic ablation causes wasting of type IIb myofibres. We show that Tw2 expression maintains progenitor cells in an undifferentiated state that is poised to initiate myogenesis in response to appropriate cues that extinguish Tw2 expression. Tw2-expressing myogenic progenitors represent a previously unrecognized, fibre-type-specific stem cell involved in postnatal muscle growth and regeneration.
AB - Skeletal muscle possesses remarkable regenerative potential due to satellite cells, an injury-responsive stem cell population located beneath the muscle basal lamina that expresses Pax7. By lineage tracing of progenitor cells expressing the Twist2 (Tw2) transcription factor in mice, we discovered a myogenic lineage that resides outside the basal lamina of adult skeletal muscle. Tw2+ progenitors are molecularly and anatomically distinct from satellite cells, are highly myogenic in vitro, and can fuse with themselves and with satellite cells. Tw2+ progenitors contribute specifically to type IIb/x myofibres during adulthood and muscle regeneration, and their genetic ablation causes wasting of type IIb myofibres. We show that Tw2 expression maintains progenitor cells in an undifferentiated state that is poised to initiate myogenesis in response to appropriate cues that extinguish Tw2 expression. Tw2-expressing myogenic progenitors represent a previously unrecognized, fibre-type-specific stem cell involved in postnatal muscle growth and regeneration.
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U2 - 10.1038/ncb3477
DO - 10.1038/ncb3477
M3 - Article
C2 - 28218909
AN - SCOPUS:85014215127
SN - 1465-7392
VL - 19
SP - 202
EP - 213
JO - Nature cell biology
JF - Nature cell biology
IS - 3
ER -