TY - JOUR
T1 - A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology
AU - Sato, Shigeo
AU - Tomomori-Sato, Chieri
AU - Parmely, Tari J.
AU - Florens, Laurence
AU - Zybailov, Boris
AU - Swanson, Selene K.
AU - Banks, Charles A.S.
AU - Jin, Jingji
AU - Cai, Yong
AU - Washburn, Michael P.
AU - Conaway, Joan Weliky
AU - Conaway, Ronald C.
N1 - Funding Information:
We thank Drs. R.G. Roeder and S. Malik for the HeLa cell line expressing FLAG-Nut2 and we thank M. Coleman for invaluable computer support. This work was supported in part by NIH Grant R37 GM41628 (to R.C.C.).
PY - 2004/6/4
Y1 - 2004/6/4
N2 - The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors. In the work described here, we exploit multidimensional protein identification technology (MudPIT) to carry out a proteomic analysis of the subunit composition of the mammalian Mediator complex. By comparing MudPIT data sets obtained from six independent Mediator preparations immunoaffinity purified through their Nut2 (MED10), Med25 (MED9), Intersex (MED29), LCMR1 (MED19), AK007855 (MED28), or CRSP70 (MED26) subunits, we identify a set of consensus mammalian Mediator subunits. In addition, we identify as Mediator-associated proteins the CDK8-like cyclin-dependent kinase CDK11 and the TRAP240-like KIAA1025 protein (MED13L), which is mutated in patients with the congenital heart defect transposition of the great arteries (TGA).
AB - The Mediator is a multiprotein transcriptional coactivator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factors. In the work described here, we exploit multidimensional protein identification technology (MudPIT) to carry out a proteomic analysis of the subunit composition of the mammalian Mediator complex. By comparing MudPIT data sets obtained from six independent Mediator preparations immunoaffinity purified through their Nut2 (MED10), Med25 (MED9), Intersex (MED29), LCMR1 (MED19), AK007855 (MED28), or CRSP70 (MED26) subunits, we identify a set of consensus mammalian Mediator subunits. In addition, we identify as Mediator-associated proteins the CDK8-like cyclin-dependent kinase CDK11 and the TRAP240-like KIAA1025 protein (MED13L), which is mutated in patients with the congenital heart defect transposition of the great arteries (TGA).
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U2 - 10.1016/j.molcel.2004.05.006
DO - 10.1016/j.molcel.2004.05.006
M3 - Article
C2 - 15175163
AN - SCOPUS:2942575993
SN - 1097-2765
VL - 14
SP - 685
EP - 691
JO - Molecular cell
JF - Molecular cell
IS - 5
ER -