TY - JOUR
T1 - A role for Ly108 in the induction of promyelocytic zinc finger transcription factor in developing thymocytes
AU - Dutta, Mala
AU - Kraus, Zachary J.
AU - Gomez-Rodriguez, Julio
AU - Hwang, Sun Hee
AU - Cannons, Jennifer L.
AU - Cheng, Jun
AU - Lee, Sang Yun
AU - Wiest, David L.
AU - Wakeland, Edward K.
AU - Schwartzberg, Pamela L.
PY - 2013/3/1
Y1 - 2013/3/1
N2 - The promyelocytic zinc finger transcription factor (PLZF) is required for the development of activated phenotypes in NKT and other innate T lymphocytes. Although strong TCR stimulation has been implicated in the induction of PLZF, the factors regulating PLZF expression are incompletely understood.We show in this study that costimulation of preselection double-positive thymocytes through the signaling lymphocyte activation molecule family receptor Ly108 markedly enhanced PLZF expression compared with that induced by TCR stimulation alone. Costimulation with Ly108 increased expression of early growth response protein (Egr)-2 and binding of Egr-2 to the promoter of Zbtb16, which encodes PLZF, and resulted in PLZF levels similar to those seen in NKT cells. In contrast, costimulation with anti-CD28 failed to enhance Egr-2 binding and Zbtb16 expression. Moreover, mice lacking Ly108 showed decreased numbers of PLZF-expressing CD4+ T cells. Together, these results support a potential role for Ly108 in the induction of PLZF.
AB - The promyelocytic zinc finger transcription factor (PLZF) is required for the development of activated phenotypes in NKT and other innate T lymphocytes. Although strong TCR stimulation has been implicated in the induction of PLZF, the factors regulating PLZF expression are incompletely understood.We show in this study that costimulation of preselection double-positive thymocytes through the signaling lymphocyte activation molecule family receptor Ly108 markedly enhanced PLZF expression compared with that induced by TCR stimulation alone. Costimulation with Ly108 increased expression of early growth response protein (Egr)-2 and binding of Egr-2 to the promoter of Zbtb16, which encodes PLZF, and resulted in PLZF levels similar to those seen in NKT cells. In contrast, costimulation with anti-CD28 failed to enhance Egr-2 binding and Zbtb16 expression. Moreover, mice lacking Ly108 showed decreased numbers of PLZF-expressing CD4+ T cells. Together, these results support a potential role for Ly108 in the induction of PLZF.
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U2 - 10.4049/jimmunol.1202145
DO - 10.4049/jimmunol.1202145
M3 - Article
C2 - 23355739
AN - SCOPUS:84874223739
SN - 0022-1767
VL - 190
SP - 2121
EP - 2128
JO - Journal of Immunology
JF - Journal of Immunology
IS - 5
ER -