TY - JOUR
T1 - A review on electroporation-based intracellular delivery
AU - Shi, Junfeng
AU - Ma, Yifan
AU - Zhu, Jing
AU - Chen, Yuanxin
AU - Sun, Yating
AU - Yao, Yicheng
AU - Yang, Zhaogang
AU - Xie, Jing
N1 - Funding Information:
This research was supported by grants from the Jilin Province Science and Technology Development Program (No. 20170414039GH) and the Special Projects of Cooperation between Jilin University and Jilin Province (No. SXGJSF2017-1-1-(10)).
Funding Information:
Funding: This research was supported by grants from the Jilin Province Science and Technology Development Program (No. 20170414039GH) and the Special Projects of Cooperation between Jilin University and Jilin Province (No. SXGJSF2017-1-1-(10)).
Publisher Copyright:
© 2018 by the authors.
PY - 2018/11/21
Y1 - 2018/11/21
N2 - Intracellular delivery is a critical step in biological discoveries and has been widely utilized in biomedical research. A variety of molecular tools have been developed for cell-based gene therapies, including FDA approved CAR-T immunotherapy, iPSC, cell reprogramming and gene editing. Despite the inspiring results of these applications, intracellular delivery of foreign molecules including nucleic acids and proteins remains challenging. Efficient yet non-invasive delivery of biomolecules in a high-throughput manner has thus long fascinates the scientific community. As one of the most popular non-viral technologies for cell transfection, electroporation has gone through enormous development with the assist of nanotechnology and microfabrication. Emergence of miniatured electroporation system brought up many merits over the weakness of traditional electroporation system, including precise dose control and high cell viability. These new generation of electroporation systems are of considerable importance to expand the biological applications of intracellular delivery, bypassing the potential safety issue of viral vectors. In this review, we will go over the recent progresses in the electroporation-based intracellular delivery and several potential applications of cutting-edge research on the miniatured electroporation, including gene therapy, cellular reprogramming and intracellular probe.
AB - Intracellular delivery is a critical step in biological discoveries and has been widely utilized in biomedical research. A variety of molecular tools have been developed for cell-based gene therapies, including FDA approved CAR-T immunotherapy, iPSC, cell reprogramming and gene editing. Despite the inspiring results of these applications, intracellular delivery of foreign molecules including nucleic acids and proteins remains challenging. Efficient yet non-invasive delivery of biomolecules in a high-throughput manner has thus long fascinates the scientific community. As one of the most popular non-viral technologies for cell transfection, electroporation has gone through enormous development with the assist of nanotechnology and microfabrication. Emergence of miniatured electroporation system brought up many merits over the weakness of traditional electroporation system, including precise dose control and high cell viability. These new generation of electroporation systems are of considerable importance to expand the biological applications of intracellular delivery, bypassing the potential safety issue of viral vectors. In this review, we will go over the recent progresses in the electroporation-based intracellular delivery and several potential applications of cutting-edge research on the miniatured electroporation, including gene therapy, cellular reprogramming and intracellular probe.
KW - Electroporation
KW - Intracellular delivery
KW - Microfabrication
KW - Miniatured electroporation
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U2 - 10.3390/molecules23113044
DO - 10.3390/molecules23113044
M3 - Review article
C2 - 30469344
AN - SCOPUS:85057099782
SN - 1420-3049
VL - 23
JO - Molecules
JF - Molecules
IS - 11
M1 - 3044
ER -