A rapid whole-mount staining procedure for nuclei of mammalian embryos

V. G. Pursel; R. J. Wall; C. E. Rexroad; Robert E Hammer; R. L. Brinster

doi:10.1016/0093-691X(85)90167-0

A rapid whole-mount staining procedure for nuclei of mammalian embryos

V. G. Pursel, R. J. Wall, C. E. Rexroad, Robert E Hammer, R. L. Brinster

Research output: Contribution to journal › Article › peer-review

150 Scopus citations

Abstract

A rapid staining procedure for examining nuclei of mammalian embryos is described. Embryos are placed on a glass slide, counterstained with trypan blue, stained with Hoechst 33342, and embedded between the slide and coverslip in Permount. The nuclei of the embryos fluoresce brightly when examined by fluorescence microscopy immediately after staining or after extended storage. The technique has proved to be an effective tool for studying the development of cow, hamster, mouse, pig, rabbit, and sheep embryos.

Original language	English (US)
Pages (from-to)	687-691
Number of pages	5
Journal	Theriogenology
Volume	24
Issue number	6
DOIs	https://doi.org/10.1016/0093-691X(85)90167-0
State	Published - Dec 1985

Keywords

Hoechst 33342
embryos
fluorescence
nuclear stain
trypan blue

ASJC Scopus subject areas

Small Animals
Food Animals
Animal Science and Zoology
Equine

Access to Document

10.1016/0093-691X(85)90167-0

Cite this

@article{7a2aefb389774988a015bcf29f77b297,

title = "A rapid whole-mount staining procedure for nuclei of mammalian embryos",

abstract = "A rapid staining procedure for examining nuclei of mammalian embryos is described. Embryos are placed on a glass slide, counterstained with trypan blue, stained with Hoechst 33342, and embedded between the slide and coverslip in Permount. The nuclei of the embryos fluoresce brightly when examined by fluorescence microscopy immediately after staining or after extended storage. The technique has proved to be an effective tool for studying the development of cow, hamster, mouse, pig, rabbit, and sheep embryos.",

keywords = "Hoechst 33342, embryos, fluorescence, nuclear stain, trypan blue",

author = "Pursel, {V. G.} and Wall, {R. J.} and Rexroad, {C. E.} and Hammer, {Robert E} and Brinster, {R. L.}",

year = "1985",

month = dec,

doi = "10.1016/0093-691X(85)90167-0",

language = "English (US)",

volume = "24",

pages = "687--691",

journal = "Theriogenology",

issn = "0093-691X",

publisher = "Elsevier Inc.",

number = "6",

}

TY - JOUR

T1 - A rapid whole-mount staining procedure for nuclei of mammalian embryos

AU - Pursel, V. G.

AU - Wall, R. J.

AU - Rexroad, C. E.

AU - Hammer, Robert E

AU - Brinster, R. L.

PY - 1985/12

Y1 - 1985/12

N2 - A rapid staining procedure for examining nuclei of mammalian embryos is described. Embryos are placed on a glass slide, counterstained with trypan blue, stained with Hoechst 33342, and embedded between the slide and coverslip in Permount. The nuclei of the embryos fluoresce brightly when examined by fluorescence microscopy immediately after staining or after extended storage. The technique has proved to be an effective tool for studying the development of cow, hamster, mouse, pig, rabbit, and sheep embryos.

AB - A rapid staining procedure for examining nuclei of mammalian embryos is described. Embryos are placed on a glass slide, counterstained with trypan blue, stained with Hoechst 33342, and embedded between the slide and coverslip in Permount. The nuclei of the embryos fluoresce brightly when examined by fluorescence microscopy immediately after staining or after extended storage. The technique has proved to be an effective tool for studying the development of cow, hamster, mouse, pig, rabbit, and sheep embryos.

KW - Hoechst 33342

KW - embryos

KW - fluorescence

KW - nuclear stain

KW - trypan blue

UR - http://www.scopus.com/inward/record.url?scp=0001244196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001244196&partnerID=8YFLogxK

U2 - 10.1016/0093-691X(85)90167-0

DO - 10.1016/0093-691X(85)90167-0

M3 - Article

AN - SCOPUS:0001244196

SN - 0093-691X

VL - 24

SP - 687

EP - 691

JO - Theriogenology

JF - Theriogenology

IS - 6

ER -

A rapid whole-mount staining procedure for nuclei of mammalian embryos

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this