A rapid whole-mount staining procedure for nuclei of mammalian embryos

V. G. Pursel, R. J. Wall, C. E. Rexroad, Robert E Hammer, R. L. Brinster

Research output: Contribution to journalArticlepeer-review

150 Scopus citations


A rapid staining procedure for examining nuclei of mammalian embryos is described. Embryos are placed on a glass slide, counterstained with trypan blue, stained with Hoechst 33342, and embedded between the slide and coverslip in Permount. The nuclei of the embryos fluoresce brightly when examined by fluorescence microscopy immediately after staining or after extended storage. The technique has proved to be an effective tool for studying the development of cow, hamster, mouse, pig, rabbit, and sheep embryos.

Original languageEnglish (US)
Pages (from-to)687-691
Number of pages5
Issue number6
StatePublished - Dec 1985


  • Hoechst 33342
  • embryos
  • fluorescence
  • nuclear stain
  • trypan blue

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine


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