A rapid assay for circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome

Ramesh Saxena; Bengt Isaksson; Per Bygren; Jörgen Wieslander

doi:10.1016/0022-1759(89)90055-0

A rapid assay for circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome

Ramesh Saxena, Bengt Isaksson, Per Bygren, Jörgen Wieslander

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

A rapid ELISA for the detection of circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome is described. The specificity of the test was shown to be highly dependent on the antigens used. Using the purified Goodpasture antigen it was possible to shorten the incubation times to 10 min in a routine assay using alkaline phosphatase-labeled second antibodies and the total assay was complete in 30 min. 200 reference sera, 500 sera from patients with various types of glomerulonephritis and 32 sera from patients with Goodpasture syndrome were analyzed by this rapid assay. The assay was able to discriminate between Goodpasture syndrome and other forms of glomerulonephritis. Using enzyme amplification it was possible to further shorten the incubation times to 1 min and the total time of the assay to 6 min.

Original language	English (US)
Pages (from-to)	73-78
Number of pages	6
Journal	Journal of Immunological Methods
Volume	118
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(89)90055-0
State	Published - Mar 10 1989

Keywords

Anti-GBM antibody
ELISA
Enzyme amplification
Goodpasture antigen

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(89)90055-0

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title = "A rapid assay for circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome",

abstract = "A rapid ELISA for the detection of circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome is described. The specificity of the test was shown to be highly dependent on the antigens used. Using the purified Goodpasture antigen it was possible to shorten the incubation times to 10 min in a routine assay using alkaline phosphatase-labeled second antibodies and the total assay was complete in 30 min. 200 reference sera, 500 sera from patients with various types of glomerulonephritis and 32 sera from patients with Goodpasture syndrome were analyzed by this rapid assay. The assay was able to discriminate between Goodpasture syndrome and other forms of glomerulonephritis. Using enzyme amplification it was possible to further shorten the incubation times to 1 min and the total time of the assay to 6 min.",

keywords = "Anti-GBM antibody, ELISA, Enzyme amplification, Goodpasture antigen",

author = "Ramesh Saxena and Bengt Isaksson and Per Bygren and J{\"o}rgen Wieslander",

note = "Funding Information: The authors wish to thank the Swedish Institute for grants to Ramesh Saxena and Ms. Katarina Danielsson and Ms. Britt Marie Karlsson for their secretarial assistance.",

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doi = "10.1016/0022-1759(89)90055-0",

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T1 - A rapid assay for circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome

AU - Saxena, Ramesh

AU - Isaksson, Bengt

AU - Bygren, Per

AU - Wieslander, Jörgen

N1 - Funding Information: The authors wish to thank the Swedish Institute for grants to Ramesh Saxena and Ms. Katarina Danielsson and Ms. Britt Marie Karlsson for their secretarial assistance.

PY - 1989/3/10

Y1 - 1989/3/10

N2 - A rapid ELISA for the detection of circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome is described. The specificity of the test was shown to be highly dependent on the antigens used. Using the purified Goodpasture antigen it was possible to shorten the incubation times to 10 min in a routine assay using alkaline phosphatase-labeled second antibodies and the total assay was complete in 30 min. 200 reference sera, 500 sera from patients with various types of glomerulonephritis and 32 sera from patients with Goodpasture syndrome were analyzed by this rapid assay. The assay was able to discriminate between Goodpasture syndrome and other forms of glomerulonephritis. Using enzyme amplification it was possible to further shorten the incubation times to 1 min and the total time of the assay to 6 min.

AB - A rapid ELISA for the detection of circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome is described. The specificity of the test was shown to be highly dependent on the antigens used. Using the purified Goodpasture antigen it was possible to shorten the incubation times to 10 min in a routine assay using alkaline phosphatase-labeled second antibodies and the total assay was complete in 30 min. 200 reference sera, 500 sera from patients with various types of glomerulonephritis and 32 sera from patients with Goodpasture syndrome were analyzed by this rapid assay. The assay was able to discriminate between Goodpasture syndrome and other forms of glomerulonephritis. Using enzyme amplification it was possible to further shorten the incubation times to 1 min and the total time of the assay to 6 min.

KW - Anti-GBM antibody

KW - ELISA

KW - Enzyme amplification

KW - Goodpasture antigen

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A rapid assay for circulating anti-glomerular basement membrane antibodies in Goodpasture syndrome

Abstract

Keywords

ASJC Scopus subject areas

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