Abstract
We present a new module integrated into the widely adopted PatternLab for proteomics to enable analysis of isotope-labeled peptides produced using dimethyl or SILAC. The accurate quantitation of proteins lies within the heart of proteomics; dimethylation has shown to be reliable, inexpensive, and applicable to any sample type. We validate our algorithm using an M. tuberculosis dataset obtained from two biological conditions; we used three dimethyl labels, one serving as an internal control for labeling a mixture of samples from both biological conditions. This internal control certified the proper functioning of our software. Availability: http://patternlabforproteomics.org, freely available for academic use.
Original language | English (US) |
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Article number | 103371 |
Journal | Journal of Proteomics |
Volume | 202 |
DOIs | |
State | Published - Jun 30 2019 |
Externally published | Yes |
Keywords
- Bioinformatics
- Differential proteomics
- Dimethyl
- PatternLab
ASJC Scopus subject areas
- Biophysics
- Biochemistry