A platform for high-throughput identification and characterization of intact proteins from Methanococcus jannaschii

Andrew J. Forbes; Jeffrey R. Johnson; Steven M. Patrie; Neil L. Kelleher

A platform for high-throughput identification and characterization of intact proteins from Methanococcus jannaschii

Andrew J. Forbes, Jeffrey R. Johnson, Steven M. Patrie, Neil L. Kelleher

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The development of general methods for identifying and characterizing intact proteins in high throughput manner, using native proteins from Methanococcus jannaschii, was discussed. A 2-D separation to sufficiently fractionate the soluble portion of the proteome, followed by automated fourier transform mass spectrometry (FTMS) and MS/MS for the proteins in these samples, with a modified THRASH algorithm were used to analyze the data. The soluble portion of the lysate was separated via preparative denatured polyacrylamide gel electrophoresis. The protein precipitation had demonstrated high recovery, and the resolution of the HPLC traces indicated that the acid-labile surfactant (ALS) was both removed or degraded by the precipitation steps.

Original language	English (US)
Title of host publication	Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	239-240
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Forbes, AJ, Johnson, JR, Patrie, SM & Kelleher, NL 2002, A platform for high-throughput identification and characterization of intact proteins from Methanococcus jannaschii. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 239-240, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

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abstract = "The development of general methods for identifying and characterizing intact proteins in high throughput manner, using native proteins from Methanococcus jannaschii, was discussed. A 2-D separation to sufficiently fractionate the soluble portion of the proteome, followed by automated fourier transform mass spectrometry (FTMS) and MS/MS for the proteins in these samples, with a modified THRASH algorithm were used to analyze the data. The soluble portion of the lysate was separated via preparative denatured polyacrylamide gel electrophoresis. The protein precipitation had demonstrated high recovery, and the resolution of the HPLC traces indicated that the acid-labile surfactant (ALS) was both removed or degraded by the precipitation steps.",

author = "Forbes, {Andrew J.} and Johnson, {Jeffrey R.} and Patrie, {Steven M.} and Kelleher, {Neil L.}",

year = "2002",

language = "English (US)",

pages = "239--240",

booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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T1 - A platform for high-throughput identification and characterization of intact proteins from Methanococcus jannaschii

AU - Forbes, Andrew J.

AU - Johnson, Jeffrey R.

AU - Patrie, Steven M.

AU - Kelleher, Neil L.

PY - 2002

Y1 - 2002

N2 - The development of general methods for identifying and characterizing intact proteins in high throughput manner, using native proteins from Methanococcus jannaschii, was discussed. A 2-D separation to sufficiently fractionate the soluble portion of the proteome, followed by automated fourier transform mass spectrometry (FTMS) and MS/MS for the proteins in these samples, with a modified THRASH algorithm were used to analyze the data. The soluble portion of the lysate was separated via preparative denatured polyacrylamide gel electrophoresis. The protein precipitation had demonstrated high recovery, and the resolution of the HPLC traces indicated that the acid-labile surfactant (ALS) was both removed or degraded by the precipitation steps.

AB - The development of general methods for identifying and characterizing intact proteins in high throughput manner, using native proteins from Methanococcus jannaschii, was discussed. A 2-D separation to sufficiently fractionate the soluble portion of the proteome, followed by automated fourier transform mass spectrometry (FTMS) and MS/MS for the proteins in these samples, with a modified THRASH algorithm were used to analyze the data. The soluble portion of the lysate was separated via preparative denatured polyacrylamide gel electrophoresis. The protein precipitation had demonstrated high recovery, and the resolution of the HPLC traces indicated that the acid-labile surfactant (ALS) was both removed or degraded by the precipitation steps.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442710440&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442710440

SP - 239

EP - 240

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

A platform for high-throughput identification and characterization of intact proteins from Methanococcus jannaschii

Abstract

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ASJC Scopus subject areas

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