A plasma proteogenomic signature for fibromuscular dysplasia

Jeffrey W. Olin, Antonio F. Di Narzo, Valentina D'Escamard, Daniella Kadian-Dodov, Haoxiang Cheng, Adrien Georges, Annette King, Allison Thomas, Temo Barwari, Katherine C. Michelis, Rihab Bouchareb, Emir Bander, Anelechi Anyanwu, Paul Stelzer, Farzan Filsoufi, Sander Florman, Mete Civelek, Stephanie Debette, Xavier Jeunemaitre, Johan L.M. BjörkegrenManuel Mayr, Nabila Bouatia-Naji, Ke Hao, Jason C. Kovacic

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Aims: Fibromuscular dysplasia (FMD) is a poorly understood disease that predominantly affects women during middle-life, with features that include stenosis, aneurysm, and dissection of medium-large arteries. Recently, plasma proteomics has emerged as an important means to understand cardiovascular diseases. Our objectives were: (i) to characterize plasma proteins and determine if any exhibit differential abundance in FMD subjects vs. matched healthy controls and (ii) to leverage these protein data to conduct systems analyses to provide biologic insights on FMD, and explore if this could be developed into a blood-based FMD test. Methods and results: Females with 'multifocal' FMD and matched healthy controls underwent clinical phenotyping, dermal biopsy, and blood draw. Using dual-capture proximity extension assay and nuclear magnetic resonance-spectroscopy, we evaluated plasma levels of 981 proteins and 31 lipid sub-classes, respectively. In a discovery cohort (Ncases = 90, Ncontrols = 100), we identified 105 proteins and 16 lipid sub-classes (predominantly triglycerides and fatty acids) with differential plasma abundance in FMD cases vs. controls. In an independent cohort (Ncases = 23, Ncontrols = 28), we successfully validated 37 plasma proteins and 10 lipid sub-classes with differential abundance. Among these, 5/37 proteins exhibited genetic control and Bayesian analyses identified 3 of these as potential upstream drivers of FMD. In a 3rd cohort (Ncases = 506, Ncontrols = 876) the genetic locus of one of these upstream disease drivers, CD2-associated protein (CD2AP), was independently validated as being associated with risk of having FMD (odds ratios = 1.36; P = 0.0003). Immune-fluorescence staining identified that CD2AP is expressed by the endothelium of medium-large arteries. Finally, machine learning trained on the discovery cohort was used to develop a test for FMD. When independently applied to the validation cohort, the test showed a c-statistic of 0.73 and sensitivity of 78.3%. Conclusion: FMD exhibits a plasma proteogenomic and lipid signature that includes potential causative disease drivers, and which holds promise for developing a blood-based test for this disease.

Original languageEnglish (US)
Pages (from-to)63-77
Number of pages15
JournalCardiovascular Research
Volume116
Issue number1
DOIs
StatePublished - Jan 1 2020
Externally publishedYes

Keywords

  • CD2AP
  • Fibromuscular dysplasia
  • Plasma protein
  • Proteomics

ASJC Scopus subject areas

  • General Medicine

Fingerprint

Dive into the research topics of 'A plasma proteogenomic signature for fibromuscular dysplasia'. Together they form a unique fingerprint.

Cite this