A new immunochemical assay for biotin

Le Phuc Thuy, L. Sweetman, W. L. Nyhan

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


A double antibody technique has been developed to separate free biotin from bound biotin after competitive binding of [3H]biotin and unlabelled biotin to avidin. Antiavidin goat antibody was added followed by the addition of antigoat IgG antibody linked to agarose. Centrifugation separated the free biotin from the biotin bound to the avidin complex. The method was suitable for the detection of the amounts of biotin contained in 100-200 /μl of plasma or 5-10 μl of urine. Normal values for the concentration of biotin in plasma and urine determined by this assay were 1.27 ± 0.67 nmol/l and 49.1 ± 35.7 μmol/mol creatinine, respectively.

Original languageEnglish (US)
Pages (from-to)191-197
Number of pages7
JournalClinica Chimica Acta
Issue number3
StatePublished - Oct 31 1991
Externally publishedYes


  • Biotin
  • Immunochemical assay

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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