A new class of scorpion toxin binding sites related to an A-type K+ channel: Pharmacological characterization and localization in rat brain

Hélène Vacher; Régine Romi-Lebrun; Christiane Mourre; Bruno Lebrun; Said Kourrich; Frédérique Masméjean; Terumi Nakajima; Christian Legros; Marcel Crest; Pierre E. Bougis; Marie France Martin-Eauclaire

doi:10.1016/S0014-5793(01)02620-5

A new class of scorpion toxin binding sites related to an A-type K⁺ channel: Pharmacological characterization and localization in rat brain

Hélène Vacher, Régine Romi-Lebrun, Christiane Mourre, Bruno Lebrun, Said Kourrich, Frédérique Masméjean, Terumi Nakajima, Christian Legros, Marcel Crest, Pierre E. Bougis, Marie France Martin-Eauclaire

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A new scorpion toxin (3751.8 Da) was isolated from the Buthus martensi venom, sequenced and chemically synthesized (sBmTX3). The A-type current of striatum neurons in culture completely disappeared when 1 μM sBmTX3 was applied (K _d=54 nM), whereas the sustained K⁺ current was unaffected. ¹²⁵I-sBmTX3 specifically bound to rat brain synaptosomes (maximum binding=14 fmol mg^-1 of protein, K _d=0.21 nM). A panel of toxins yet described as specific ligands for K⁺ channels were unable to compete with ¹²⁵I-sBmTX3. A high density of ¹²⁵I-sBmTX3 binding sites was found in the striatum, hippocampus, superior colliculus, and cerebellum in the adult rat brain.

Original language	English (US)
Pages (from-to)	31-36
Number of pages	6
Journal	FEBS Letters
Volume	501
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(01)02620-5
State	Published - Jul 13 2001

Keywords

A-type K current
Distribution in rat brain
Scorpion toxin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(01)02620-5

Cite this

Vacher, H., Romi-Lebrun, R., Mourre, C., Lebrun, B., Kourrich, S., Masméjean, F., Nakajima, T., Legros, C., Crest, M., Bougis, P. E., & Martin-Eauclaire, M. F. (2001). A new class of scorpion toxin binding sites related to an A-type K⁺ channel: Pharmacological characterization and localization in rat brain. FEBS Letters, 501(1), 31-36. https://doi.org/10.1016/S0014-5793(01)02620-5

Vacher, H, Romi-Lebrun, R, Mourre, C, Lebrun, B, Kourrich, S, Masméjean, F, Nakajima, T, Legros, C, Crest, M, Bougis, PE & Martin-Eauclaire, MF 2001, 'A new class of scorpion toxin binding sites related to an A-type K⁺ channel: Pharmacological characterization and localization in rat brain', FEBS Letters, vol. 501, no. 1, pp. 31-36. https://doi.org/10.1016/S0014-5793(01)02620-5

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abstract = "A new scorpion toxin (3751.8 Da) was isolated from the Buthus martensi venom, sequenced and chemically synthesized (sBmTX3). The A-type current of striatum neurons in culture completely disappeared when 1 μM sBmTX3 was applied (K d=54 nM), whereas the sustained K+ current was unaffected. 125I-sBmTX3 specifically bound to rat brain synaptosomes (maximum binding=14 fmol mg-1 of protein, K d=0.21 nM). A panel of toxins yet described as specific ligands for K+ channels were unable to compete with 125I-sBmTX3. A high density of 125I-sBmTX3 binding sites was found in the striatum, hippocampus, superior colliculus, and cerebellum in the adult rat brain.",

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note = "Funding Information: We thank Dr. P. Marchot for providing purified α-DTX and G. Jacquet for helpful in patch-clamp experiments. This study was supported by a grant from the DGA to H.V. Publisher Copyright: {\textcopyright} 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.",

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AU - Crest, Marcel

AU - Bougis, Pierre E.

AU - Martin-Eauclaire, Marie France

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