@inbook{5da58260ffe54478993ef065f34f1962,
title = "A Method for Orthotopic Transplantation of Lung Cancer in Mice",
abstract = "Preclinical mouse models of lung cancer have been vital experimental tools to elucidate cancer biology and test novel therapeutic regimens. Two main models are most commonly used—genetically engineered mouse models and xenograft transplantation models. The most common xenograft model employs subcutaneous transplantation of tumor cells. However, the subcutaneous space is a foreign environment to lung cancer cells and does not appropriately model the tumor-stromal interactions of endogenous lung cancers. Here, we present an orthotopic mouse model of lung cancer that utilizes direct injection of cancer cells into the lung parenchyma that allows many potential studies including interactions of lung fibroblast Hedgehog pathway activity and tumor epithelia. The protocol describes this procedure and its potential applications for lung cancer research.",
keywords = "Intrathoracic injections, Lung cancer, Mouse, Orthotopic transplantation, Tumor microenvironment, Xenograft",
author = "Wilson, {Alexandra N.} and Baozhi Chen and Xin Liu and Kurie, {Jonathan M.} and James Kim",
note = "Funding Information: The work is supported in part by University of Texas Southwestern, National Cancer Institute (1R01CA196851), the Department of Defense (W81XWH-14-1-0338), American Cancer Society (ACS-IRG-02-196), and the Sidney Kimmel Foundation for Cancer Research (SKF-14-057) to JK. Alexandra N. Wilson and Baozhi Chen contributed equally to this work. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1701-4_20",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "231--242",
booktitle = "Methods in Molecular Biology",
}