A Method for Orthotopic Transplantation of Lung Cancer in Mice

Alexandra N. Wilson, Baozhi Chen, Xin Liu, Jonathan M. Kurie, James Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Preclinical mouse models of lung cancer have been vital experimental tools to elucidate cancer biology and test novel therapeutic regimens. Two main models are most commonly used—genetically engineered mouse models and xenograft transplantation models. The most common xenograft model employs subcutaneous transplantation of tumor cells. However, the subcutaneous space is a foreign environment to lung cancer cells and does not appropriately model the tumor-stromal interactions of endogenous lung cancers. Here, we present an orthotopic mouse model of lung cancer that utilizes direct injection of cancer cells into the lung parenchyma that allows many potential studies including interactions of lung fibroblast Hedgehog pathway activity and tumor epithelia. The protocol describes this procedure and its potential applications for lung cancer research.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Intrathoracic injections
  • Lung cancer
  • Mouse
  • Orthotopic transplantation
  • Tumor microenvironment
  • Xenograft

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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