A mammalian chromatin remodeling complex with similarities to the yeast INO80 complex

Jingji Jin, Yong Cai, Tingting Yao, Aaron J. Gottschalk, Laurence Florens, Selene K. Swanson, José L. Gutiérrez, Michael K. Coleman, Jerry L. Workman, Arcady Mushegian, Michael P. Washburn, Ronald C. Conaway, Joan Weliky Conaway

Research output: Contribution to journalArticlepeer-review

191 Scopus citations


The mammalian Tip49a and Tip49b proteins belong to an evolutionarily conserved family of AAA+ ATPases. In Saccharomyces cerevisiae, orthologs of Tip49a and Tip49b, called Rvb1 and Rvb2, respectively, are subunits of two distinct ATP-dependent chromatin remodeling complexes, SWR1 and INO80. We recently demonstrated that the mammalian Tip49a and Tip49b proteins are integral subunits of a chromatin remodeling complex bearing striking similarities to the S, cerevisiae SWR1 complex (Cai, Y., Jin, J., Florens, L., Swanson, S. K., Kusch, T., Li, B., Workman, J. L., Washburn, M. P., Conaway, R. C., and Conaway, J. W. (2005) J. Biol. Chem. 280, 13665-13670). In this report, we identify a new mammalian Tip49a- and Tip49b-containing ATP-dependent chromatin remodeling complex, which includes orthologs of 8 of the 15 subunits of the S. cerevisiae INO80 chromatin remodeling complex as well as at least five additional subunits unique to the human INO80 (hINO80) complex. Finally, we demonstrate that, similar to the yeast INO80 complex, the hINO80 complex exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.

Original languageEnglish (US)
Pages (from-to)41207-41212
Number of pages6
JournalJournal of Biological Chemistry
Issue number50
StatePublished - Dec 16 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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