TY - JOUR
T1 - A macrophage factor inhibits adipocyte gene expression
T2 - An in vitro model of cachexia
AU - Torti, Frank M.
AU - Dieckmann, Barbara
AU - Beutler, Bruce
AU - Cerami, Anthony
AU - Ringold, Gordon M.
PY - 1985
Y1 - 1985
N2 - Certain infections and malignancies in mammals cause the development of a condition known as cachexia in which the animal continues to lose weight, often while consuming an adequate diet. When macrophages are stimulated with an endotoxin, they produce a factor or factors, termed cachectin, that inhibits the activity of fat-producing (lipogenic) enzymes in cultured adipocytes. This effect may reflect one of the physiological bases for cachexia. In the present study, clones of complementary DNA from genes whose expression is increased during the differentiation of adipocytes were used to study the molecular basis of cachectin's actions. In the presence of cachectin, the expression of the corresponding genes was reversibly and specifically inhibited. Furthermore, when mature adipocytes were exposed to cachectin, the messenger RNA's of those genes diminished and rapidly approached the levels present before differentiation.
AB - Certain infections and malignancies in mammals cause the development of a condition known as cachexia in which the animal continues to lose weight, often while consuming an adequate diet. When macrophages are stimulated with an endotoxin, they produce a factor or factors, termed cachectin, that inhibits the activity of fat-producing (lipogenic) enzymes in cultured adipocytes. This effect may reflect one of the physiological bases for cachexia. In the present study, clones of complementary DNA from genes whose expression is increased during the differentiation of adipocytes were used to study the molecular basis of cachectin's actions. In the presence of cachectin, the expression of the corresponding genes was reversibly and specifically inhibited. Furthermore, when mature adipocytes were exposed to cachectin, the messenger RNA's of those genes diminished and rapidly approached the levels present before differentiation.
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U2 - 10.1126/science.3839597
DO - 10.1126/science.3839597
M3 - Article
C2 - 3839597
AN - SCOPUS:0022385114
SN - 0036-8075
VL - 229
SP - 867
EP - 869
JO - Science
JF - Science
IS - 4716
ER -