A lyophilized formulation of RiVax, a recombinant ricin subunit vaccine, retains immunogenicity

Joan E. Smallshaw; Ellen S. Vitetta

doi:10.1016/j.vaccine.2009.12.081

A lyophilized formulation of RiVax, a recombinant ricin subunit vaccine, retains immunogenicity

Joan E. Smallshaw, Ellen S. Vitetta

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Ricin is a CDC level B biothreat. Our recombinant ricin A chain vaccine (RiVax) contains two mutations, rendering it non-toxic at high doses. Frozen or alum formulations of RiVax protected mice against ricin administered by injection, gavage or aerosol. Without alum, RiVax was safe and immunogenic in rabbits and human volunteers. For military use, the predominant target group, it would be optimal not to require a cold chain for transport and storage. We have now developed a lyophilized formulation and demonstrated stability and efficacy for at least 1 year stored refrigerated or at room temperature administered with or without alum.

Original language	English (US)
Pages (from-to)	2428-2435
Number of pages	8
Journal	Vaccine
Volume	28
Issue number	12
DOIs	https://doi.org/10.1016/j.vaccine.2009.12.081
State	Published - Mar 11 2010

Keywords

Lyophilization
Ricin
Vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2009.12.081

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abstract = "Ricin is a CDC level B biothreat. Our recombinant ricin A chain vaccine (RiVax) contains two mutations, rendering it non-toxic at high doses. Frozen or alum formulations of RiVax protected mice against ricin administered by injection, gavage or aerosol. Without alum, RiVax was safe and immunogenic in rabbits and human volunteers. For military use, the predominant target group, it would be optimal not to require a cold chain for transport and storage. We have now developed a lyophilized formulation and demonstrated stability and efficacy for at least 1 year stored refrigerated or at room temperature administered with or without alum.",

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AU - Smallshaw, Joan E.

AU - Vitetta, Ellen S.

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AB - Ricin is a CDC level B biothreat. Our recombinant ricin A chain vaccine (RiVax) contains two mutations, rendering it non-toxic at high doses. Frozen or alum formulations of RiVax protected mice against ricin administered by injection, gavage or aerosol. Without alum, RiVax was safe and immunogenic in rabbits and human volunteers. For military use, the predominant target group, it would be optimal not to require a cold chain for transport and storage. We have now developed a lyophilized formulation and demonstrated stability and efficacy for at least 1 year stored refrigerated or at room temperature administered with or without alum.

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KW - Vaccine

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A lyophilized formulation of RiVax, a recombinant ricin subunit vaccine, retains immunogenicity

Abstract

Keywords

ASJC Scopus subject areas

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