TY - JOUR
T1 - A “hot Spot”-Enhanced paper lateral flow assay for ultrasensitive detection of traumatic brain injury biomarker S-100β in blood plasma
AU - Gao, Xuefei
AU - Boryczka, Jennifer
AU - Zheng, Peng
AU - Kasani, Sujan
AU - Yang, Feng
AU - Engler-Chiurazzi, Elizabeth B.
AU - Simpkins, James W.
AU - Wigginton, Jane G.
AU - Wu, Nianqiang
N1 - Funding Information:
This work was partially supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health ( R15NS087515 ). The views and options in this article presented in this article do not necessarily reflect those of NIH.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - Currently colorimetric paper lateral flow strips (PLFS) encounter two major limitations, that is, low sensitivity and severe interference from complex sample matrices such as blood. These shortcomings limit their application in detection of low-concentration analytes in complex samples. To solve these problems, a PLFS has been developed by utilizing surface-enhanced Raman scattering (SERS) for sensing signal transduction. In particular, a hierarchical three-dimensional nanostructure has been designed to create “hot spots”, which can significantly amplify the SERS sensing signal, leading to high sensitivity. As a result, this PLFS has demonstrated a limit of detection (LOD) of 5.0 pg mL−1 toward detection of S-100β, a traumatic brain injury (TBI) protein biomarker in blood plasma. The PLFS has been successfully used for rapid measurement of S-100β in clinical TBI patient samples taken in the emergency department. Availability of PLFS for blood testing would shift the paradigm of TBI patient management and clinical outcome in emergency departments. It is expected that this type of PLFS can be adapted for rapid detection of various human diseases due to its capability of measuring a low level of protein blood biomarkers in complex human fluids.
AB - Currently colorimetric paper lateral flow strips (PLFS) encounter two major limitations, that is, low sensitivity and severe interference from complex sample matrices such as blood. These shortcomings limit their application in detection of low-concentration analytes in complex samples. To solve these problems, a PLFS has been developed by utilizing surface-enhanced Raman scattering (SERS) for sensing signal transduction. In particular, a hierarchical three-dimensional nanostructure has been designed to create “hot spots”, which can significantly amplify the SERS sensing signal, leading to high sensitivity. As a result, this PLFS has demonstrated a limit of detection (LOD) of 5.0 pg mL−1 toward detection of S-100β, a traumatic brain injury (TBI) protein biomarker in blood plasma. The PLFS has been successfully used for rapid measurement of S-100β in clinical TBI patient samples taken in the emergency department. Availability of PLFS for blood testing would shift the paradigm of TBI patient management and clinical outcome in emergency departments. It is expected that this type of PLFS can be adapted for rapid detection of various human diseases due to its capability of measuring a low level of protein blood biomarkers in complex human fluids.
KW - Biomarker
KW - Blood testing
KW - Paper lateral flow assay
KW - S-100β
KW - Surface-enhanced Raman scattering
KW - Traumatic brain injury
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U2 - 10.1016/j.bios.2021.112967
DO - 10.1016/j.bios.2021.112967
M3 - Article
C2 - 33429202
AN - SCOPUS:85099003049
SN - 0956-5663
VL - 177
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 112967
ER -