A Gja1 missense mutation in a mouse model of oculodentodigital dysplasia

Ann M. Flenniken, Lucy R. Osborne, Nicole Anderson, Nadia Ciliberti, Craig Fleming, Joanne E.I. Gittens, Xiang Qun Gong, Lois B. Kelsey, Crystal Lounsbury, Luisa Moreno, Brian J. Nieman, Katie Peterson, Dawei Qu, Wendi Roscoe, Qing Shao, Dan Tong, Gregory I.L. Veitch, Irina Voronina, Igor Vukobradovic, Geoffrey A. WoodYonghong Zhu, Ralph A. Zirngibl, Jane E. Aubin, Donglin Bai, Benoit G. Bruneau, Marc Grynpas, Janet E. Henderson, R. Mark Henkelman, Colin McKerlie, John G. Sled, William L. Stanford, Dale W. Laird, Gerald M. Kidder, S. Lee Adamson, Janet Rossant

Research output: Contribution to journalArticlepeer-review

211 Scopus citations


Oculodentodigital dysplasia (ODDD) is an autosomal dominant disorder characterized by pleiotropic developmental anomalies of the limbs, teeth, face and eyes that was shown recently to be caused by mutations in the gap junction protein alpha 1 gene (GJA1), encoding connexin 43 (Cx43). In the course of performing an N-ethyl-N-nitrosourea mutagenesis screen, we identified a dominant mouse mutation that exhibits many classic symptoms of ODDD, including syndactyly, enamel hypoplasia, craniofacial anomalies and cardiac dysfunction. Positional cloning revealed that these mice carry a point mutation in Gja1 leading to the substitution of a highly conserved amino acid (G60S) in Cx43. In vivo and in vitro studies revealed that the mutant Cx43 protein acts in a dominant-negative fashion to disrupt gap junction assembly and function. In addition to the classic features of ODDD, these mutant mice also showed decreased bone mass and mechanical strength, as well as altered hematopoietic stem cell and progenitor populations. Thus, these mice represent an experimental model with which to explore the clinical manifestations of ODDD and to evaluate potential intervention strategies.

Original languageEnglish (US)
Pages (from-to)4375-4386
Number of pages12
Issue number19
StatePublished - Oct 2005
Externally publishedYes


  • Connexin 43
  • Missense mutation
  • Mouse model
  • Oculodentodigital dysplasia

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


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