Abstract
Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG10883).
Original language | English (US) |
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Pages (from-to) | 11880-11885 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 103 |
Issue number | 32 |
DOIs | |
State | Published - Aug 8 2006 |
Keywords
- Posttranscriptional silencing
- RNAi screen
- dsRNA
- siRNA
- siRNA pathway
ASJC Scopus subject areas
- General