A direct radioimmunoassay of corticosterone in rat serum

C. Gomez Sanchez; B. A. Murry; D. C. Kem; Norman M Kaplan

doi:10.1210/endo-96-3-796

A direct radioimmunoassay of corticosterone in rat serum

C. Gomez Sanchez, B. A. Murry, D. C. Kem, Norman M Kaplan

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Abstract

A direct radioimmunoassay of cortico-sterone is described requiring as little as 1–2 μl of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.

Original language	English (US)
Pages (from-to)	796-798
Number of pages	3
Journal	Endocrinology
Volume	96
Issue number	3
DOIs	https://doi.org/10.1210/endo-96-3-796
State	Published - Mar 1975

ASJC Scopus subject areas

Endocrinology

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title = "A direct radioimmunoassay of corticosterone in rat serum",

abstract = "A direct radioimmunoassay of cortico-sterone is described requiring as little as 1–2 μl of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.",

author = "{Gomez Sanchez}, C. and Murry, {B. A.} and Kem, {D. C.} and Kaplan, {Norman M}",

year = "1975",

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AU - Kem, D. C.

AU - Kaplan, Norman M

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N2 - A direct radioimmunoassay of cortico-sterone is described requiring as little as 1–2 μl of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.

AB - A direct radioimmunoassay of cortico-sterone is described requiring as little as 1–2 μl of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.

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A direct radioimmunoassay of corticosterone in rat serum

Abstract

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