@article{2c66e8999dcd41caba17d2400c531c9c,
title = "A Dimeric Structural Scaffold for PRC2-PCL Targeting to CpG Island Chromatin",
abstract = "PRC2 dimerization has long been noted, with unclear functions. Chen et al. reveal the structural basis of PRC2 dimerization in the context of PRC2-PCL (PRC2.1), which is drastically different from PRC2-AEBP2 (PRC2.2) in structural architecture. PRC2 dimerization controls CGI DNA binding in vitro and H3K27me3 deposition in vivo, possibly through an avidity effect.",
keywords = "CpG island (CGI), chromatin, crystal structure, epigenetics, gene silencing, histone methylation, polycomb repressive complex 2 (PRC2), polycomb-like (PCL), protein dimerization, structural biology",
author = "Siming Chen and Lianying Jiao and Xiuli Liu and Xin Yang and Xin Liu",
note = "Funding Information: The SUZ12 KO mESC line was a gift from Dr. Kristian Helin at Memorial Sloan Kettering Cancer Center. The cDNA of EPOP was kindly provided by Dr. Yang Shi at Harvard Medical School and Children{\textquoteright}s Hospital Boston. SEC-MALS was performed with the help of Dr. Chad Brautigam from the Macromolecular Biophysics Resource at UT Southwestern Medical Center. This research was supported by Welch Foundation research grant I-1790 , CPRIT research grant R1119 , a Rita Allen Foundation research grant, and NIH grants GM114576 and GM121662 (to Xin Liu). Xin Liu is a W.W. Caruth, Jr., Scholar in Biomedical Research. This research also received support from the Cecil H. and Ida Green Center Training Program in Reproductive Biology Sciences Research . This project was supported by the Protein and Monoclonal Antibody Production Shared Resource at Baylor College of Medicine with funding from NIH Cancer Center Support Grant P30 CA125123 . This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under contract no. DE-AC02-06CH11357. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences , of the U.S. DOE under contract no. DE-AC02-05CH11231 . Use of the Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under contract DE-AC02-76SF00515 . The SSRL Structural Molecular Biology Program is supported by the DOE Office of Biological and Environmental Research and the NIH National Institute of General Medical Sciences (including P41GM103393 ). The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of NIGMS or NIH. Funding Information: The SUZ12 KO mESC line was a gift from Dr. Kristian Helin at Memorial Sloan Kettering Cancer Center. The cDNA of EPOP was kindly provided by Dr. Yang Shi at Harvard Medical School and Children's Hospital Boston. SEC-MALS was performed with the help of Dr. Chad Brautigam from the Macromolecular Biophysics Resource at UT Southwestern Medical Center. This research was supported by Welch Foundation research grant I-1790, CPRIT research grant R1119, a Rita Allen Foundation research grant, and NIH grants GM114576 and GM121662 (to Xin Liu). Xin Liu is a W.W. Caruth, Jr. Scholar in Biomedical Research. This research also received support from the Cecil H. and Ida Green Center Training Program in Reproductive Biology Sciences Research. This project was supported by the Protein and Monoclonal Antibody Production Shared Resource at Baylor College of Medicine with funding from NIH Cancer Center Support Grant P30 CA125123. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under contract no. DE-AC02-06CH11357. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. DOE under contract no. DE-AC02-05CH11231. Use of the Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under contract DE-AC02-76SF00515. The SSRL Structural Molecular Biology Program is supported by the DOE Office of Biological and Environmental Research and the NIH National Institute of General Medical Sciences (including P41GM103393). The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of NIGMS or NIH. S.C. and Xin Liu conceived the study and designed the experiments. S.C. performed the experiments with assistance from L.J. Xiuli Liu, and X.Y. S.C. and L.J. acquired the crystallographic data and built the structural model. S.C. L.J. and Xin Liu wrote the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2020",
month = mar,
day = "19",
doi = "10.1016/j.molcel.2019.12.019",
language = "English (US)",
volume = "77",
pages = "1265--1278.e7",
journal = "Molecular cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "6",
}