A detergent-free method for purifying caveolae membrane from tissue culture cells

Eric J. Smart; Yun Shu Ying; Chieko Mineo; Richard G W Anderson

doi:10.1073/pnas.92.22.10104

A detergent-free method for purifying caveolae membrane from tissue culture cells

Eric J. Smart, Yun Shu Ying, Chieko Mineo, Richard G W Anderson

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676 Scopus citations

Abstract

Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.

Original language	English (US)
Pages (from-to)	10104-10108
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	92
Issue number	22
DOIs	https://doi.org/10.1073/pnas.92.22.10104
State	Published - Oct 24 1995

Keywords

G proteins
epidermal growth factor receptor
folate receptor

ASJC Scopus subject areas

General

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10.1073/pnas.92.22.10104

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abstract = "Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.",

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AU - Ying, Yun Shu

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AU - Anderson, Richard G W

PY - 1995/10/24

Y1 - 1995/10/24

N2 - Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.

AB - Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.

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KW - epidermal growth factor receptor

KW - folate receptor

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A detergent-free method for purifying caveolae membrane from tissue culture cells

Abstract

Keywords

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