TY - JOUR
T1 - A cytolytic human T lymphocyte clone differentially recognizing HLA-B27 subtypes
AU - Miyachi, Yoshiki
AU - Wee, Siew Lin
AU - Chen, Li Kuang
AU - Grumet, F. Carl
AU - Bowman, Robert J.
AU - Taurog, Joel D.
N1 - Funding Information:
This work was supported by a grant from the Kroc Foundation to Dr. Taurog and by a grant from the Minnesota Chapter of the Arthritis Foundation. Dr. Miyachi was supported by the Kroc Foundation during the tenure of this work. Dr. Wee is a Special Fellow of the Leukemia Society of America and is supported by NIH grants AI 17687, AI 19007, and AM 13083. Dr. Chen is supported by the Ministry of National Defense of the Republic of China. Dr. Taurog is supported by NIH Young Investigator Award AM 29799. The authors wish to thank Dr. Fritz H. Bach for many helpful discussions and for his enthusiastic support. We also thank Drs. Nancy Meryhew and Malcolm Blumenthal for providing HLA-typed families and other donors; Dr. Roger Colton for permitting us to study several of his patients; Dr. Janice Nicklas for providing LCL HLA loss variants; the staffs of the St. Paul Red Cross and the University of Minnesota Tissue Typing Laboratory for their extensive support and cheerful cooperation; Mr. Andrew Watson for providing monoclonal antibodies; and Ms. Barbara Bulbulian, Ms. Mary Sue Dierckins, and Mr. Gregory Sandberg for technical assistance.
PY - 1984/8
Y1 - 1984/8
N2 - A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27-; 2) six families, including 20 B27+ and 14 B27- individuals; and 3) B27+ and B27- variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30-104%, while lysis of B27+,B27M2- targets was 4-22%. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19%, while lysis of all other B27 - targets was ≤10%. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis.
AB - A cytolytic human T cell (CTL) clone, designated F/M-F159, has been produced, the lytic specificity of which distinguishes subtypes of HLA-B27. This was demonstrated in cell-mediated lympholysis (CML) assays of: 1) a panel of target cells from unrelated donors, 75 B27 + and 36 B27-; 2) six families, including 20 B27+ and 14 B27- individuals; and 3) B27+ and B27- variants of a B27+ lymphoblastoid cell line (LCL). Specificity of F/M-F159 for HLA-B27 was confirmed by blocking studies with monoclonal antibodies. Lysis of B27+ targets reactive with the anti-B27 monoclonal antibody B27M2 was 30-104%, while lysis of B27+,B27M2- targets was 4-22%. Lysis of B27- targets expressing HLA-Bw47, known to be cross-reactive with the B27M2 antibody, was 10 to 19%, while lysis of all other B27 - targets was ≤10%. Clone F/M-F159 lysed B27+ targets, and failed to lyse B27-targets, irrespective of the clinical status of the cell donors. It is concluded that F/M-F159 recognizes an epitope present on the majority of serologically identified HLA-B27 molecules and that this epitope is close related to, but not identical with, the epitope recognized by the antibody B27M2. These findings are interpreted as supporting a direct role for HLA-B27 in disease pathogenesis.
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U2 - 10.1016/0198-8859(84)90089-2
DO - 10.1016/0198-8859(84)90089-2
M3 - Article
C2 - 6206036
AN - SCOPUS:0021593349
SN - 0198-8859
VL - 10
SP - 237
EP - 249
JO - Human Immunology
JF - Human Immunology
IS - 4
ER -