Abstract
2-Hydroxyglutarate (2HG) is produced in gliomas with mutations of isocitrate dehydrogenase (IDH) 1 and 2. The 1H resonances of the J-coupled spins of 2HG are extensively overlapped with signals from other metabolites. Here, we report a comparative study at 3 T of the utility of the point-resolved spectroscopy sequence with a standard short TE (35 ms) and a long TE (97 ms), which had been theoretically designed for the detection of the 2HG 2.25-ppm resonance. The performance of the methods is evaluated using data from phantoms, seven healthy volunteers and 22 subjects with IDH-mutated gliomas. The results indicate that TE=97 ms provides higher detectability of 2HG than TE=35 ms, and that this improved capability is gained when data are analyzed with basis spectra that include the effects of the volume localizing radiofrequency and gradient pulses.
Original language | English (US) |
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Pages (from-to) | 1242-1250 |
Number of pages | 9 |
Journal | NMR in biomedicine |
Volume | 26 |
Issue number | 10 |
DOIs | |
State | Published - Oct 2013 |
Keywords
- 2-hydroxyglutarate (2HG)
- 3 T
- H MRS
- Human brain
- IDH-mutated gliomas
- Point-resolved spectroscopy (PRESS)
- Short/long TE
- Volume-localized simulations
ASJC Scopus subject areas
- Molecular Medicine
- Radiology Nuclear Medicine and imaging
- Spectroscopy