A comparative study of choline acetyltransferase from normal and Alzheimer brain

Gordon Bruce

doi:10.1016/0304-3940(85)90195-8

A comparative study of choline acetyltransferase from normal and Alzheimer brain

Gordon Bruce

Pediatrics

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Abstract

A comparitive study was made of the enzyme choline acetyltransferase (ChAT) from normal and Alzheimer (senile dementia of the Alzheimer type) brain. The number of molecular weight and charge forms of the enzyme were determined in the caudate region of both brains. Efficient purification of active ChAT was achieved using immuno-affinity purification. It was shown that the purified enzyme was identical in both cases, exhibiting a single charge (apparent pI∼8.2) and a single molecular weight (mol. wt. = 68,000). The idea of a selective loss of one particular isoform to explain the reduced levels of ChAT observed in Alzheimer's disease can be ruled out.

Original language	English (US)
Pages (from-to)	171-175
Number of pages	5
Journal	Neuroscience letters
Volume	59
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(85)90195-8
State	Published - Aug 30 1985

Keywords

Alzheimer's disease
choline acetyltransferase
multiple forms

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(85)90195-8

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AB - A comparitive study was made of the enzyme choline acetyltransferase (ChAT) from normal and Alzheimer (senile dementia of the Alzheimer type) brain. The number of molecular weight and charge forms of the enzyme were determined in the caudate region of both brains. Efficient purification of active ChAT was achieved using immuno-affinity purification. It was shown that the purified enzyme was identical in both cases, exhibiting a single charge (apparent pI∼8.2) and a single molecular weight (mol. wt. = 68,000). The idea of a selective loss of one particular isoform to explain the reduced levels of ChAT observed in Alzheimer's disease can be ruled out.

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A comparative study of choline acetyltransferase from normal and Alzheimer brain

Abstract

Keywords

ASJC Scopus subject areas

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