Abstract
4-Hydroxycinnamoyl-CoA hydratase/lyase (HCHL), a crotonase homologue of phenylpropanoid catabolism from Pseudomonas fluorescens strain AN103, led to the formation of 4-hydroxybenzaldehyde metabolites when expressed in hairy root cultures of Datura stramonium L. established by transformation with Agrobacterium rhizogenes. The principal new compounds observed were the glucoside and glucose ester of 4-hydroxybenzoic acid, together with 4-hydroxybenzyl alcohol-O-β-D-glucoside. In lines actively expressing HCHL, these together amounted to around 0.5% of tissue fresh mass. No protocatechuic derivatives were found, although a trace of vanillic acid-β-D-glucoside was detected. There was no accumulation of 4-hydroxybenzaldehydes, whether free or in the form of their glucose conjugates. There was some evidence suggesting a diminished availability of feruloyl-CoA for the production of feruloyl putrescine and coniferyl alcohol. The findings are discussed in the context of a diversion of phenylpropanoid metabolism, and the ability of plants and plant cultures to conjugate phenolic compounds.
Original language | English (US) |
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Pages (from-to) | 79-89 |
Number of pages | 11 |
Journal | Planta |
Volume | 215 |
Issue number | 1 |
DOIs | |
State | Published - 2002 |
Keywords
- 4-Hydroxycinnamoyl-CoA hydratase/lyase
- Datura (phenylpropanoid metabolism)
- Glucoside
- Hydroxybenzaldehyde
- Phenylpropanoid metabolism
- Vanillin
ASJC Scopus subject areas
- Genetics
- Plant Science