TY - JOUR
T1 - [27] B Cell Growth and Differentiation Factors
AU - Brooks, K.
AU - Fernandez, R.
AU - Vitetta, E. S.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - This chapter provides an overview of B cell growth and their differentiation factors. The growth and differentiation of B lymphocytes is mediated by soluble products released by T cells and macrophages. B cell trophic cytokines include interleukin-1 (IL-1) which is secreted by macrophages, B cell stimulatory factor I (BSF-1; previously known as B cell growth factor I or BCGF I), B cell growth factor II (BCGF II), T cell replacing factor (TRF), interleukin-2 (IL-2), and several B cell differentiation and maturation factors (BCDF or BMF) which are secreted by T cells. The chapter further discusses preparation of supernatants containing BSF-1. BSF-l-containing supernatants are derived from T cells stimulated either with mitogens, or phorbol myristate acetate (PMA). The initial step in purifying BSF-1 involves concentration of crude supernatants by ammonium sulfate precipitation. The bulk of the BSF-1 activity is found in the 50-100% saturated ammonium sulfate fractions. The next purification step varies among laboratories but involves either Phenyl Sepharose chromatography. In addition, BCGF II is also described with its purification.
AB - This chapter provides an overview of B cell growth and their differentiation factors. The growth and differentiation of B lymphocytes is mediated by soluble products released by T cells and macrophages. B cell trophic cytokines include interleukin-1 (IL-1) which is secreted by macrophages, B cell stimulatory factor I (BSF-1; previously known as B cell growth factor I or BCGF I), B cell growth factor II (BCGF II), T cell replacing factor (TRF), interleukin-2 (IL-2), and several B cell differentiation and maturation factors (BCDF or BMF) which are secreted by T cells. The chapter further discusses preparation of supernatants containing BSF-1. BSF-l-containing supernatants are derived from T cells stimulated either with mitogens, or phorbol myristate acetate (PMA). The initial step in purifying BSF-1 involves concentration of crude supernatants by ammonium sulfate precipitation. The bulk of the BSF-1 activity is found in the 50-100% saturated ammonium sulfate fractions. The next purification step varies among laboratories but involves either Phenyl Sepharose chromatography. In addition, BCGF II is also described with its purification.
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U2 - 10.1016/S0076-6879(85)16029-5
DO - 10.1016/S0076-6879(85)16029-5
M3 - Article
C2 - 3937026
AN - SCOPUS:0022272546
SN - 0076-6879
VL - 116
SP - 372
EP - 379
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -